Differential cadmium translocation and accumulation between Nicotiana tabacum L. and Nicotiana rustica L. by transcriptome combined with chemical form analyses
نویسندگان
چکیده
منابع مشابه
Coproporphyrinogenase in tobacco (Nicotiana tabacum L.).
1. Coproporphyrinogenase was extracted and purified from tobacco (Nicotiana tabacum L.). Enzyme activity was mainly located in mitochondria rather than in chloroplasts. The enzyme was purified by differential centrifugation, ammonium sulphate fractionation, calcium phosphate gel adsorption and dialysis. A 69-fold final purification was obtained. 2. An apparent K(m) value of 3.6x10(-5)m was foun...
متن کاملCarbohydrate-limited Growth Kinetics of Tobacco (Nicotiana rustica L.) Callus.
Logistic curves were fitted to sigmoidal growth data obtained from tobacco (Nicotiana rustica L.) callus grown on media prepared with 0.1, 0.03, 0.01, and 0.003 m sucrose. Analysis of the growth curves indicated that final yields and specific growth rates were influenced by the initial sucrose concentration. Growth yields from the four treatments were similar (0.61 +/- 0.04 gram dry tissue per ...
متن کاملDifferential Ion Stimulation of Plasmalemma Adenosine Triphosphatase from Leaf Epidermis and Mesophyll of Nicotiana rustica L.
An ATPase preparation, presumed to be associated with plasma membrane due to the coincidence on isopycnic gradients of cellulase and beta-glucan synthetase at high substrate, has been isolated from the epidermal and mesophyll of tobacco leaf. The ATPase from both tissues was found to prefer ATP over other nucleotides. The pH optimum was 7.0 in the presence of 3 millimolar MgCl(2) and pH 6.5 in ...
متن کاملAcylated Steryl Glycoside Synthesis in Seedlings of Nicotiana tabacum L.
In tobacco seedlings (Nicotiana tabacum L.), glucose from supplied uridine diphosphate-[U-(14)C]glucose was first incorporated into steryl glycosides and later into acylated steryl glycosides. However, when [(14)C]cholesterol was used as substrate, the acylated steryl glycosides became labeled earlier than the steryl glycosides. With [(14)C]cholesteryl glucoside as substrate, most of the radioa...
متن کاملSteryl Glycoside Formation in Seedlings of Nicotiana tabacum L.
Particulate enzyme preparations from tobacco seedlings (Nicotiana tabacum L.) were used in the synthesis of steryl glycoside. The data obtained by measuring cholesterol-4-(14)C incorporation generally agree with results obtained with UDP-glucose-(14)C. The in vitro reaction was linear for the first 10 minutes and had a pH optimum of 7.0 to 7.4. Addition of ATP activated while UDP-glucose inhibi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Ecotoxicology and Environmental Safety
سال: 2021
ISSN: 0147-6513
DOI: 10.1016/j.ecoenv.2020.111412